Laser Flash Photolysis
Description: An excitation technique used to study the reactions of transient species in chemical and biological systems
Recommended Product: Quantel Brilliant
LKS.60 Laser Flash Photolysis system - picture courtesy of Applied Photophysics
Laser Flash Photolysis is one of the most effective methods for studying the reactions of transient species such as radicals, excited states or ions, in chemical and biological systems.
A typical Laser Flash Photolysis spectrometer will consist of a nanosecond pulsed laser (pump), a xenon lamp (probe) and a spectrometer. The laser pump source provides a single wavelength excitation with nanosecond resolution and generates transient chemical and biological species in the sample. These excited states, radicals and ions undergo chemical and physical interactions and hence their varying absorption characteristics can be directly observed. The varying absorbtion characteristics are measured via the xenon lamp and spectrometer.
This technique is compatible with liquid, gaseous, and solid samples and is now widely used in bio-chemical research, such as electron transport in cytochromes or protein folding.