WHY IS SEM IMAGING USED TO STUDY NANOFIBRES?
SEM imaging is an ideal tool to check the fibre surface properties and the structure of collagen networks. In general, a lot of organic and biological samples such as tissue, blood vessels and cartilage provide very interesting information when imaged by SEM. Such materials however are very poor electrical and thermal conductors. Hence, they tend to charge heavily when they are scanned by the electron beam in an electron microscope. We used the membrane of an inner egg shell as a source of collagen to investigate the charging in an electron microscope. We noticed that an uncoated collagen sample needed charges, even a relatively low magnifications. Hence, metal coating was determined as the best solution to image collagen.
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